This has been the most exciting two weeks so far in the duration of this project. This is because all of my materials arrived, so I was able to start to assemble my experiment. This also meant that all of my knowledge about my experiment and my procedure because I needed to properly compile all of my materials and place everything with intention and purpose.
NBT-II, Nara Bladder Tumor No. 2, currently our most promising cell line since receiving news that the RT-4 cell line is not a possibility and any other human or primate-derived cell line. NBT-II is a tumor that is derived from the urinary bladder of a male rat. Although it is not RT-4 which is an epithelial bladder cancer cell line derived from a 63-year-old caucasian male and was the initial cell line I was planning on using. One major issue, regardless of the cell line I use, is how I am going to store the cells without liquid nitrogen when I am on breaks. This is because many cell lines require liquid nitrogen as a means of storage over lengthier periods of time (weeks).
These two weeks have been a bit of a change. Instead of looking at different research articles and various YouTube tutorials, I was actually able to make this vision start to come to life with the addition of the first round of materials. This means that the next phase of my project is beginning.
Graph 1.1 Change in total fertility rate of the global average, France, and Germany, from 1960 to 2016, data obtained from World Bank Open Data (“Fertility rate”).
In this blog post, I have carried out a comparative analysis of the fertility rates of Germany and France, which have traditionally been compared to each other in the field of birth rate research. I was initially going to conduct research on Germany alone, however, I decided to make a change in my plan for two reasons: to learn what makes France an appropriate target of comparison to Germany, and to see whether comparing different countries allows me to identify the unique characteristics of each country in a more effective manner.
In this blog post, I would explore medicine during the High Middle Ages by using a similar format as that of my previous blog post, which focused on three general regions–Europe, the Middle East and China.
Medicine during the High Middle Ages
The expansion and consolidation of power of the kings of France, Spain and England gradually stabilized Europe, restoring vitality to medicine and other fields of study. Continue reading
Approval has been granted! After nearly a year of paperwork being filled out and troubleshooting, Westtown has officially been granted access to Biosafety Level 1 products (as long as they are not derived from humans or primates). This is a great step forward because I essentially have all of the approval needed in order to begin my actual research.
The past two weeks has been a relatively kinetic time span in terms of planning out the logistics of this project. I say this because there have been a lot of different things/ideas that have come into light that are taking this project to new heights.
Throughout the past weeks, I have once again attempted to receive approval for purchasing biosafety level one products by submitting my application to ATCC once again. Biosafety level one, is the lowest level of the four biosafety and is work that, typically, involve microbes with low rates of affecting the health of people or infecting them. While waiting for approval from ATCC, I have also reached out to an employee at a company called Morphotek in hopes of gaining some local assistance in the process of cell culturing and problem solving should any issues arise throughout the process of my research. Continue reading
Medicine during the Middle Ages
Similar to the cycles in an economy, the development of modern western medicine has never been a straight line. Influenced by cultural beliefs, political climate and the general global dynamics, medicine since the demise of the Western Roman Empire to the Renaissance period went through abrupt halts and astonishing accelerations. Continue reading