Tag Archives: science

Managing Algal Growth and Plan Moving Forward – Nick

    The last two weeks have been pretty slow compared to the rest of the time in the experiment. This is because the main focus of the past weeks have mainly been algae control and tank cleanliness. This has been difficult to maintain because the local aquarium does not have any snails in stock.

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Genitourinary Cancer Research: a new problem

eve

Over the course of the past week, we ran multiple tests on the viability of the cells using NanoEntek’s cell counter. We were hoping for the 20% viability of the cells, previously measured, to rise to an ideal 70%. One hypothesis was that the cell counter was not working properly, however, after upon review in the inverted microscope (manual cell counting), we determined that the cells were far too small to be seen even under 40x magnification.

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Introducing the Coral Frags into the Tanks – Nick

   Ever since the first semester, there has been a lot of work going on both inside and outside of the classroom. Whether it’s calling Zack, cleaning the tanks, completely emptying and resetting the tank, or even just doing regular water tests. There has also been a lot of roadblocks in the process of setting up the experiment.

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Managing the Tanks Over Break and Returning from Break – Nick

      I was extremely excited over break as well as my return because of how much the experiment would progress. The next phase of the project was organizing the stabilization of the water in the tanks, figuring out the specifics on our actual coral specimens, then we had to sort out how we are going to set up the platform in which we are going to place the corals, and we had to finalize plans on the lighting fixture.

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Genitourinary Cancer Research: a setback – Dhillon

wcuSeal

(University Seal [Photograph]. (n.d.). Retrieved from West Chester University
database)

I thought it would be smooth sailing after Thanksgiving break, and I could begin my research. However, after meeting with T. Mariska who brought into light some issues. We have decided to push back research till after Winter break. During our meeting, we discussed possibilities regarding storage as well as the length it would take to culture my cells. After further discussions, we have established that it may be best for me to begin research after winter break. This is because of the short turnaround between Thanksgiving and Winter Break. The estimated cost of the needed FBS (Fetal Bovine Serum), NBT-ii cell line, and the medium is about $1,189.00.

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Assembling My Experiment and Beginning Water Stabilization -Nick

This has been the most exciting two weeks so far in the duration of this project. This is because all of my materials arrived, so I was able to start to assemble my experiment. This also meant that all of my knowledge about my experiment and my procedure because I needed to properly compile all of my materials and place everything with intention and purpose.

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Genitourinary Cancer​: NBT-II -Dhillon

NBT-II, Nara Bladder Tumor No. 2, currently our most promising cell line since receiving news that the RT-4 cell line is not a possibility and any other human or primate-derived cell line. NBT-II is a tumor that is derived from the urinary bladder of a male rat. Although it is not RT-4 which is an epithelial bladder cancer cell line derived from a 63-year-old caucasian male and was the initial cell line I was planning on using. One major issue, regardless of the cell line I use, is how I am going to store the cells without liquid nitrogen when I am on breaks. This is because many cell lines require liquid nitrogen as a means of storage over lengthier periods of time (weeks).

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Gathering Materials and Planning Setup – Nick

   These two weeks have been a bit of a change. Instead of looking at different research articles and various YouTube tutorials, I was actually able to make this vision start to come to life with the addition of the first round of materials. This means that the next phase of my project is beginning.

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