Over the course of the past week, we ran multiple tests on the viability of the cells using NanoEntek’s cell counter. We were hoping for the 20% viability of the cells, previously measured, to rise to an ideal 70%. One hypothesis was that the cell counter was not working properly, however, after upon review in the inverted microscope (manual cell counting), we determined that the cells were far too small to be seen even under 40x magnification.
(University Seal [Photograph]. (n.d.). Retrieved from West Chester University
I thought it would be smooth sailing after Thanksgiving break, and I could begin my research. However, after meeting with T. Mariska who brought into light some issues. We have decided to push back research till after Winter break. During our meeting, we discussed possibilities regarding storage as well as the length it would take to culture my cells. After further discussions, we have established that it may be best for me to begin research after winter break. This is because of the short turnaround between Thanksgiving and Winter Break. The estimated cost of the needed FBS (Fetal Bovine Serum), NBT-ii cell line, and the medium is about $1,189.00.
NBT-II, Nara Bladder Tumor No. 2, currently our most promising cell line since receiving news that the RT-4 cell line is not a possibility and any other human or primate-derived cell line. NBT-II is a tumor that is derived from the urinary bladder of a male rat. Although it is not RT-4 which is an epithelial bladder cancer cell line derived from a 63-year-old caucasian male and was the initial cell line I was planning on using. One major issue, regardless of the cell line I use, is how I am going to store the cells without liquid nitrogen when I am on breaks. This is because many cell lines require liquid nitrogen as a means of storage over lengthier periods of time (weeks).
Approval has been granted! After nearly a year of paperwork being filled out and troubleshooting, Westtown has officially been granted access to Biosafety Level 1 products (as long as they are not derived from humans or primates). This is a great step forward because I essentially have all of the approval needed in order to begin my actual research.