The last two weeks have been pretty slow compared to the rest of the time in the experiment. This is because the main focus of the past weeks have mainly been algae control and tank cleanliness. This has been difficult to maintain because the local aquarium does not have any snails in stock.
Throughout the past few weeks, I have been in contact with both ATCC and NanoEnTek regarding the NBT-II cell line. After further analyzation of the protocol provided to me by ATCC technical support and cross-comparison with my own procedures carried out, it was clear that two steps were not carried out. The first instance was not washing the cell lines in Dulbecco Phosphate Buffered Saline. DPBS removes excess serum left behind from medium and inhibits or lengthen the trypsinization period possibly preventing the adherent cells from completely detaching from the bottom of the T-75 flasks. Additionally, during the initial subculturing carried out I was supposed to centrifuge the complete growth medium from the T-75 flask at 125xg for 5-7 minutes and then resuspend these cells in fresh media as an additional culture to keep going. Additionally, I will be purchasing more of the Eve NanoEnTek cell counting slides to further test the viability of my new cells following my initial culturing.
These two weeks have completely turned my project around. This is because there was a lot of forward progress and positive results. However, there is still a lot to accomplish still before I can start the experiment.
In my last blog, I talked about my plan for the semester, why I want to study abstract algebra, and some basic concepts involving mapping. In this blog, I will first provide some update on Modeling the Future, a team math competition we have been working on, and how I redefine addition in group theory. I will also talk about my plan for the science fair. Continue reading →
Over the course of the past week, we ran multiple tests on the viability of the cells using NanoEntek’s cell counter. We were hoping for the 20% viability of the cells, previously measured, to rise to an ideal 70%. One hypothesis was that the cell counter was not working properly, however, after upon review in the inverted microscope (manual cell counting), we determined that the cells were far too small to be seen even under 40x magnification.
Ever since the first semester, there has been a lot of work going on both inside and outside of the classroom. Whether it’s calling Zack, cleaning the tanks, completely emptying and resetting the tank, or even just doing regular water tests. There has also been a lot of roadblocks in the process of setting up the experiment.
Hello there! My name is Andy Chen, a current Senior at Westtown school. This semester, I plan to take an independent science course to furthers my interests in Cognitive Science. Let’s get started!
So first, what is Cognitive Science?
When Cognitive Science first emerged in the late 1900s, scientists were unsure about its domain: was it “Cognitive Psychology under a new name,” or “an offshoot of artificial intelligence?” (Estes, 1991, p. 282). Continue reading →
I began the process of my research by culturing the complete growth medium for the initial culturing of the cells and future subculturing of the cells. Additionally, the cell line, NBT-ii, has come from ATCC and I have cultured it under the guidance of Teacher Leslie. Continue reading →
In this blog, I would like to briefly review my Fall semester and share my plan for the Spring.
In the past four months, I worked on Differential Equations and its related topics. Through following the MIT Open Course Ware, I learned different methods to solve DEs and their implications in real life.
I was extremely excited over break as well as my return because of how much the experiment would progress. The next phase of the project was organizing the stabilization of the water in the tanks, figuring out the specifics on our actual coral specimens, then we had to sort out how we are going to set up the platform in which we are going to place the corals, and we had to finalize plans on the lighting fixture.