Genitourinary Cancer Research: Cell Culturing

Image result for nbt-ii cells

(image of what the NBT-ii cells should look like)

We received cells the 4th a carried out the initial culturing protocol established. We incubated the medium in the 37 degrees 5% CO2 incubator for about 20 minutes in order to let it reach the necessary temperature. We thawed the vile of cells and carried out the remained of the steps in a sterile hood.

The next day I analyzed the cells under and inverted microscope to discover that they were contaminated. T. Mariska assisted me in what procedures I should carry out (since I had never dealt with something like this before). The contaminant was dark and rod-shaped and moved extremely quickly. We took the medium we used and placed it in the incubator – 4 days have passed since we placed the medium in the incubator and there are no signs of the contaminant. This most likely means that the cells we received may have initially been contaminated.

In my initial conversation on Friday with a representative from technical support at ATCC they said that they check all of their products for contamination and do not refund people for their products for contamination issues. I have contacted ATCC and filled out the form in order to hopefully get this issue resolved.

This process has been very frustrating and disheartening for me because I have not been even able to begin the actual research that I initially planned on conducting due to varying issues with initial and subculturing of my cell line. I am not sure how much time I will have to conduct research and what the possibilities for my research will be from this point now due to the continual issues with the culturing of my cells.

 

2 thoughts on “Genitourinary Cancer Research: Cell Culturing

  1. bessgoldstein

    Dhillon-

    I’m sorry to hear about this process you’re going through, it must be difficult dealing with these issues with time constraints. I really hope things start looking up, and you get as much research done as you can. You have been doing a great job so far, and I’m looking forward to seeing what you can hopefully come up with.
    -Bess

    Reply
  2. baitingz

    Hi Dhillon, I understand that you have went through a long way to get your cells ready, and I feel absolutely sorry that you had a hard time getting and nurturing the cells. If I am facing the same situation, I would feel the same despair, even worse! However, I want to encourage you to continue your research, which I believe you will definitely do! Furthermore, I think you may want to look for information online to see if any people met the same problems. In addition, maybe you can start to read more relevant papers. The most important thing for cancer study is certainly experiment because we don’t completely understand what are going on yet, but I think reading about other’s experiments will also help you later! I am looking forward to reading about your research on these papers!

    Reply

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