Genitourinary Cancer Research: a new problem

eve

Over the course of the past week, we ran multiple tests on the viability of the cells using NanoEntek’s cell counter. We were hoping for the 20% viability of the cells, previously measured, to rise to an ideal 70%. One hypothesis was that the cell counter was not working properly, however, after upon review in the inverted microscope (manual cell counting), we determined that the cells were far too small to be seen even under 40x magnification.

The cells however continually multiplied, whether it was our initial NBT-ii cell line or another organism that multiplied we do not know. This was apparent through observation of the cloudiness of the complete growth medium inside of the T-75 flasks. Morphology-of-M-NBT-II-versus-E-NBT-II-A-E-NBT-II-cells-and-B-M-NBT-II-cells-culture.png

(Image of what the NBT-ii cells should look like)

The cloudiness of the media after multiple days typically shows that the cells are ready for splitting/subculturing meaning that they are dividng and splitting. Because of these factors I contacted, NanoEntek’s United States office and asked if the cell counting procedures I was carrying out were correct. They proceeded to reach out to their Hong Kong offices, to answer my questions. It soon became clear that I was carrying out the proper procedures in the cell counter and the viability of my cells was very low. As a result of the lack of viability of my cells I reached out to ATCC’s technical support where they later sent out a form to explain the procedures I carried out to initially culture my cells, subculture my cells, the issue I am having with my cell line, and the storage conditions of my cells. I am hoping that they will send a new NBT-ii cell line to me, so as to avert the additional $500.00 cost to repurchase the cell line. However, if I do not hear back from ATCC regarding this, I will purchase a new cell line so as to not postpone my research any further.

 

2 thoughts on “Genitourinary Cancer Research: a new problem

  1. bessgoldstein

    Dhillion-

    I’m sorry to hear about the setback you enocuntered when observing these cells. I find it very interetsing that these cancer cells you were observing were so small to be seen even from under a microscope. You were very clear in the writing of your post, but I am unclear about what your’e doing with this problem as you move forward in the project. I understand that you are purchasing a new cell line–but for poeple who do not understand some biological/scientific terms (me), maybe it would be best to explain what that all means.

    Thank you for updating us, and looking forward to see what you will come up with.
    -Bess

    Reply
  2. baitingz

    Dhillion, congrats on starting your experiments. I am not sure if this is doable at Westtown, but I am really curious about how your cells look like in comparison to what they suppose to look like. You said that they are too small to be visible, but are continually multiplying, so do you know the reason for this to happen? Furthermore, I agree with Bess that maybe you should remind us about your primary goals, as even though it is very obvious to you, we as readers may not understand them very well. I would recommend you to talk little more about why the current situation isn’t good enough for you to accomplish your project. Also, I like how you are trying different ways to solve this problem, by reaching out to the company and by presumably purchasing other cells. Please keep up with your passion!!!

    Reply

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